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pp7 stem-loops 8×pp7  (Addgene inc)


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    Addgene inc pp7 stem-loops 8×pp7
    Schematic of components and plasmid combinations for typical SunRISER mRNA-labeling experiments (A) mRNA and protein components for SunRISER labeling experiments. (B) Schematics of SunRISER variants SRv.1 (top), SRv.1.1 (center), and SRv.1.2 (bottom) for fluorescence signal amplification. The mRNA transcript (black) is tagged at 3′ UTR with <t>PP7</t> stem loops (blue). In the first stage of signal amplification, each stem loop can be bound by two PCP coat proteins (yellow) fused to a SunTag GCN4 peptide array (orange). In the second stage of signal amplification, GFP (green) is recruited through antibody-peptide-specific binding between scFv (gray) and GCN4 epitopes. (C) Plasmid maps of the SunRISER two-plasmid (2P) variants consisting of detection plasmids (left) and protein plasmids for SRv.1-2P (top), SRv.1.1-2P (center), and SRv.1.2-2P (bottom). Using the indicated plasmids from Addgene, the GOI CDS for SRv.1 (top, left) is CFP and the GOI CDS for SRv.1.1 and SRv.1.2 (center and bottom, left) is mCherry. Although the 2P variants of SunRISER are simpler to work with, SunRISER variants using three plasmids as described previously in can be used as outlined previously and in <xref ref-type=Table 1 . " width="250" height="auto" />
    Pp7 Stem Loops 8×Pp7, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pp7 stem-loops 8×pp7/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    pp7 stem-loops 8×pp7 - by Bioz Stars, 2026-06
    90/100 stars

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    1) Product Images from "Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO"

    Article Title: Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2022.101630

    Schematic of components and plasmid combinations for typical SunRISER mRNA-labeling experiments (A) mRNA and protein components for SunRISER labeling experiments. (B) Schematics of SunRISER variants SRv.1 (top), SRv.1.1 (center), and SRv.1.2 (bottom) for fluorescence signal amplification. The mRNA transcript (black) is tagged at 3′ UTR with PP7 stem loops (blue). In the first stage of signal amplification, each stem loop can be bound by two PCP coat proteins (yellow) fused to a SunTag GCN4 peptide array (orange). In the second stage of signal amplification, GFP (green) is recruited through antibody-peptide-specific binding between scFv (gray) and GCN4 epitopes. (C) Plasmid maps of the SunRISER two-plasmid (2P) variants consisting of detection plasmids (left) and protein plasmids for SRv.1-2P (top), SRv.1.1-2P (center), and SRv.1.2-2P (bottom). Using the indicated plasmids from Addgene, the GOI CDS for SRv.1 (top, left) is CFP and the GOI CDS for SRv.1.1 and SRv.1.2 (center and bottom, left) is mCherry. Although the 2P variants of SunRISER are simpler to work with, SunRISER variants using three plasmids as described previously in can be used as outlined previously and in <xref ref-type=Table 1 . " title="... transcript (black) is tagged at 3′ UTR with PP7 stem loops (blue). In the first stage of ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Schematic of components and plasmid combinations for typical SunRISER mRNA-labeling experiments (A) mRNA and protein components for SunRISER labeling experiments. (B) Schematics of SunRISER variants SRv.1 (top), SRv.1.1 (center), and SRv.1.2 (bottom) for fluorescence signal amplification. The mRNA transcript (black) is tagged at 3′ UTR with PP7 stem loops (blue). In the first stage of signal amplification, each stem loop can be bound by two PCP coat proteins (yellow) fused to a SunTag GCN4 peptide array (orange). In the second stage of signal amplification, GFP (green) is recruited through antibody-peptide-specific binding between scFv (gray) and GCN4 epitopes. (C) Plasmid maps of the SunRISER two-plasmid (2P) variants consisting of detection plasmids (left) and protein plasmids for SRv.1-2P (top), SRv.1.1-2P (center), and SRv.1.2-2P (bottom). Using the indicated plasmids from Addgene, the GOI CDS for SRv.1 (top, left) is CFP and the GOI CDS for SRv.1.1 and SRv.1.2 (center and bottom, left) is mCherry. Although the 2P variants of SunRISER are simpler to work with, SunRISER variants using three plasmids as described previously in can be used as outlined previously and in Table 1 .

    Techniques Used: Plasmid Preparation, Labeling, Fluorescence, Amplification, Peptide Microarray, Binding Assay



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    pp7 stem-loops 8×pp7  (Addgene inc)
    90
    Addgene inc pp7 stem-loops 8×pp7
    Schematic of components and plasmid combinations for typical SunRISER mRNA-labeling experiments (A) mRNA and protein components for SunRISER labeling experiments. (B) Schematics of SunRISER variants SRv.1 (top), SRv.1.1 (center), and SRv.1.2 (bottom) for fluorescence signal amplification. The mRNA transcript (black) is tagged at 3′ UTR with <t>PP7</t> stem loops (blue). In the first stage of signal amplification, each stem loop can be bound by two PCP coat proteins (yellow) fused to a SunTag GCN4 peptide array (orange). In the second stage of signal amplification, GFP (green) is recruited through antibody-peptide-specific binding between scFv (gray) and GCN4 epitopes. (C) Plasmid maps of the SunRISER two-plasmid (2P) variants consisting of detection plasmids (left) and protein plasmids for SRv.1-2P (top), SRv.1.1-2P (center), and SRv.1.2-2P (bottom). Using the indicated plasmids from Addgene, the GOI CDS for SRv.1 (top, left) is CFP and the GOI CDS for SRv.1.1 and SRv.1.2 (center and bottom, left) is mCherry. Although the 2P variants of SunRISER are simpler to work with, SunRISER variants using three plasmids as described previously in can be used as outlined previously and in <xref ref-type=Table 1 . " width="250" height="auto" />
    Pp7 Stem Loops 8×Pp7, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pp7 stem-loops 8×pp7/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    pp7 stem-loops 8×pp7 - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

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    Schematic of components and plasmid combinations for typical SunRISER mRNA-labeling experiments (A) mRNA and protein components for SunRISER labeling experiments. (B) Schematics of SunRISER variants SRv.1 (top), SRv.1.1 (center), and SRv.1.2 (bottom) for fluorescence signal amplification. The mRNA transcript (black) is tagged at 3′ UTR with PP7 stem loops (blue). In the first stage of signal amplification, each stem loop can be bound by two PCP coat proteins (yellow) fused to a SunTag GCN4 peptide array (orange). In the second stage of signal amplification, GFP (green) is recruited through antibody-peptide-specific binding between scFv (gray) and GCN4 epitopes. (C) Plasmid maps of the SunRISER two-plasmid (2P) variants consisting of detection plasmids (left) and protein plasmids for SRv.1-2P (top), SRv.1.1-2P (center), and SRv.1.2-2P (bottom). Using the indicated plasmids from Addgene, the GOI CDS for SRv.1 (top, left) is CFP and the GOI CDS for SRv.1.1 and SRv.1.2 (center and bottom, left) is mCherry. Although the 2P variants of SunRISER are simpler to work with, SunRISER variants using three plasmids as described previously in can be used as outlined previously and in <xref ref-type=Table 1 . " width="100%" height="100%">

    Journal: STAR Protocols

    Article Title: Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO

    doi: 10.1016/j.xpro.2022.101630

    Figure Lengend Snippet: Schematic of components and plasmid combinations for typical SunRISER mRNA-labeling experiments (A) mRNA and protein components for SunRISER labeling experiments. (B) Schematics of SunRISER variants SRv.1 (top), SRv.1.1 (center), and SRv.1.2 (bottom) for fluorescence signal amplification. The mRNA transcript (black) is tagged at 3′ UTR with PP7 stem loops (blue). In the first stage of signal amplification, each stem loop can be bound by two PCP coat proteins (yellow) fused to a SunTag GCN4 peptide array (orange). In the second stage of signal amplification, GFP (green) is recruited through antibody-peptide-specific binding between scFv (gray) and GCN4 epitopes. (C) Plasmid maps of the SunRISER two-plasmid (2P) variants consisting of detection plasmids (left) and protein plasmids for SRv.1-2P (top), SRv.1.1-2P (center), and SRv.1.2-2P (bottom). Using the indicated plasmids from Addgene, the GOI CDS for SRv.1 (top, left) is CFP and the GOI CDS for SRv.1.1 and SRv.1.2 (center and bottom, left) is mCherry. Although the 2P variants of SunRISER are simpler to work with, SunRISER variants using three plasmids as described previously in can be used as outlined previously and in Table 1 .

    Article Snippet: Among the SunRISER plasmid toolkit, we provide 2 different stem-loop array lengths of PP7 stem-loops (8×PP7, 10×PP7), and plasmids for 24×PP7 are also available (Addgene #40652).

    Techniques: Plasmid Preparation, Labeling, Fluorescence, Amplification, Peptide Microarray, Binding Assay